Chromosome Microarray Service

As a trusted supplier in the field of microarrays, CD Genomics is committed to designing and developing various effective biological microarray products and providing corresponding testing services. Our microarray detection platform provides a full range of microarray-based analysis services to help our customers reduce costs while using advanced technologies for related research. We are confident to provide you with high-quality products and high-quality services to accelerate the output of research results.

Chromosome Analysis Technology

The development and application of genomic microarray technology have created a new era of clinical genetics and broke the situation of G-banding chromosome analysis as the standard for diagnosing chromosomal abnormalities in the past 30 years. Chromosome microarray analysis (CMA) provides convenient and rapid detection for the most common chromosomal diseases and a large number of serious genetic diseases that cannot be detected by traditional chromosome analysis. Early chromosome analysis detects chromosome number and structure abnormalities by counting chromosomes and analyzing chromosome band patterns in metaphase cells, while CMA detects chromosome loss and increase in the entire genome by comparing the strength of hybridization between sample DNA and control DNA. The chromosome microarray consists of small fragments of DNA probes from each of the 46 chromosomes that are known to be located. CMA can look for chromosomal imbalance and determine whether there is a difference in copy number, that is, the increase (duplication) or loss (deletion) of a particular piece of DNA. FISH of metaphase chromosomes, combined with SNP arrays, array CGH, or whole-genome sequencing, can help identify abnormal genomes after chromosome fragmentation and other types of complex genome rearrangements (abnormalities beyond the resolution of chromosome analysis).

Fig 1. Complex genomic rearrangements. (Leibowitz M L, et al. 2015)

Service

Chromosome rearrangements are generally considered to be gradually accumulated over multiple generations, that is, multiple rearrangements appear at the same time. The most significant example is chromosome fragmentation, which is characterized by tens or hundreds of rearrangements confined to a single chromosome or localized regions of a few chromosomes. Genomic analysis of chromosome fragmentation and the exploration of its biological mechanism are helpful to understand how chromosome segregation errors produce mutations and karyotype changes. Our chromosome microarray service is not limited to chromosome microarray, but also provides SNP analysis and FISH analysis services, which can detect the presence. Our services include CMA testing using more than 1.9 million copy number probes and approximately 750,000 single nucleotide polymorphism probes to detect copy number changes and over-homozygous regions. High resolution copy number variation (CNV) analysis, SNP analysis combined with CMA testing to find extra (duplication) or deletion (deletion) chromosome segments (including microdeletion and microduplication of chromosome fragments) to detect loss of heterozygosity (AOH) and uniparental disomy (UPD). In addition, CMA can also detect excessive homozygous (implying the risk of recessive diseases or imprinting disorders), triploids and other duplications of the entire genome. Identifying overly homozygous regions on a single chromosome may indicate uniparental disomy (UPD), which may require further clinical research when viewed on chromosomes with known imprinting disorders associated with UPD. In addition, the detection of excessive homozygosity on multiple chromosomes may indicate blood relationships, so it can be used to determine candidate genes to further detect autosomal recessive genetic diseases. We will give a detailed bioinformatics report, and can also provide guidance for the corresponding data analysis.

CD Genomics provides high-quality microarray tools and convenient detection services for chromosome analysis. We have a large team of scientists with a solid academic foundation and cooperate with researchers in basic biology and biomedical projects from all over the world. The advantage of choosing an analytical testing outsourcing testing service is that there is no need to consider any other costs (such as equipment and laboratory space), and it saves supervision or management. This advanced technology can quickly turn around and provide reliable results, thereby saving valuable time and resources. Please contact us for more detailed information.

References

1. MacKinnon R N. Analysis of Chromothripsis by Combined FISH and Microarray Analysis[M]//Chromothripsis. Humana Press, New York, NY, 2018: 53-77.
2. Leibowitz M L, Zhang C Z, Pellman D. Chromothripsis: a new mechanism for rapid karyotype evolution[J]. Annual review of genetics, 2015, 49: 183-211.