CD Genomics is a leading global company who can provide the best solution for worldwide customers in the field of RNA microarray. We have successfully accomplished many projects in circRNA Microarray. We guarantee the finest results for our customers all over the world.
In recent years, non-coding RNA regulation has become a hot research area, especially in disease research. Circular RNA (circRNA) is a new type of RNA, which is different from traditional linear RNA1. It has a closed ring structure and exists in a large number of eukaryotic transcriptomes. It is also a research hotspot in the field of life science and medicine. Because of the lack of 5'and 3' terminals, circRNA is more difficult to degrade than linear RNA, so it is abundant in cells and tissues. Its tissue specificity, disease specificity, sequence specificity, and high stability make circRNA a useful biomarker of clinical diseases. Recent studies have shown that circRNA plays a role as a miRNA sponge in different species, called competitive endogenous RNA (ceRNA), which can competently bind to miRNAs. The interaction with disease-related miRNAs indicates that circRNA plays an important role in disease regulation2,3. In addition, some intron circular RNA (ciRNA) promotes the transcription of host genes. circRNA has a specific expression pattern in many diseases and performs an important biological function. The expression of circRNA in diseases is the first step to study the function of circRNA.
Microarrays are very effective for detecting genome-wide circRNA4. Compared with second-generation sequencing, microarrays can detect more circRNAs, including circRNA with low expression abundance. Sequencing is easier to detect coding genes with high expression abundance than circRNA with low abundance. Conventional sequencing depths can lead to loss of circRNA abundance in many areas. Since the microarray is targeted at the back-spliced junction, all circRNA expression can be detected in theory. In microarray experiments, linear sequence amplification was used, and sequence preference of library amplification in sequencing was not observed. Microarrays have no preference for gene expression abundances and have high sensitivity and accuracy for circRNA with low expression abundances. The number of circRNAs detected in microarrays is more than in sequencing. In microsamples, if there is a small amount of exogenous sequence interference, the amplification of sequencing libraries will significantly amplify it, interfering with later data analysis. However, the microarray can be detected by specific probes without such interference. In addition, circRNA forms covalently closed ring structure through a back-splicing site, which determines the method to detect circRN. Experiments and analysis must be carried out around reverse splicing sites in sequencing and microarray. According to the published literature, conventional RNA sequencing only covers about 0.1% of the reverse splicing sites, so the efficiency of sequencing for detecting circRNA is rather low. The detection of less circRNA will limit further research on circRNA in disease or physiological state. The detection of circRNA can only be determined by detecting the specific reverse splicing sites of circRNA. Microarray probes can directly detect reverse splicing sites, so microarray detection results can almost be used for circRNA analysis, with high detection efficiency. Of course, the microarrays can mainly detect circRNAs known at the time. If new circRNA is to be found, sequencing is a more suitable platform.
In order to better study circRNA, CD Genomics developed circRNA microarrays. The sources of circRNA microarrays are integrated with the landmark literature of circRNA research. All circRNAs have been rigorously verified by experiments, which makes it possible to systematically study circRNA transcriptomes under different physiological and pathological conditions. At the same time, we labeled all circRNAs with high matching value at the target sites of miRNAs, which would be helpful for the study of the function of circRNA as a natural miRNA sponge. Our circRNA microarray covers circRNA sites from humans and mice. Each circRNA corresponds to a splicing point probe, which can detect a single circRNA accurately and reliably, and has high specificity even in the presence of linear RNA. Random primer reverse labeling system and RNase R pretreatment of samples ensured the efficient and specific labeling of circRNA. In addition, the setting of RNA spike-in control can monitor the efficiency of sample labeling and chip hybridization very well.
RNA sample requirements
- Purity: OD 260/280 should be 1.9 ~ 2.2 and DNA should be clearly removed;
- Integrity: RNA integrity number (RIN) (≥7) and 28S/18S (≥0.7);
- Concentration: no less than 100ng/µl;
- Amount: no less than 2µg;
- Preservation and transportation: RNA is stored in freezing tubes and transported with dry ice or liquid nitrogen.
CD Genomics offers high-quality services in RNA Microarray. With well-equipped instruments and experienced scientist team, we are dedicated to collaborating with our clients around the world to meet your specific requirements. We have comprehensive capacity and capabilities to provide a broad and integrated portfolio of laboratory and manufacturing services. If you are interested in our services, please contact us for more details.
References
- Amaresh C, et al. (2017). "High-puritycircular RNA isolation method (RPAD) reveals vast collection of introniccircRNAs." Nucleic Acids Research.
- Luka B et al. (2017). "Circular RNAs: Biogenesis, Function, and a Role as Possible Cancer Biomarkers." International Journal of Genomics, 1-19.
- Barrett SP et al. (2016). "Circular RNAs: Analysis, expression and potential functions." Development, 143(11), 1838-1847.
- Li S, et al. (2018). "Microarray is an efficient tool for circRNA profiling." Briefings in Bioinformatics.
