CD Genomics is a leading global company who can provide the best solution for worldwide customers in the field of DNA microarray. We have successfully accomplished many projects in Human Exome Microarray. We guarantee the finest results for our customers all over the world.

Human exome microarray is a new genome-wide level detection DNA chip. It can recognize the expression of each exon in all transcripts. Therefore, exome microarrays can detect variable alternative splicing and differential genes simultaneously. Alternative splicing is closely related to the formation of tumors¹. Therefore, alternative splicing plays an important role in studying the mechanism of diseases. Traditional expression profiling chips cover only the 3'end of the RNA, and can not detect the changes of alternative splicing and PolyA of 3' end. The exome microarray expression profile covers every exon. For each exon, corresponding probes are designed to detect the expression of each exon, so as to the purpose of detecting various changes of mRNA. Exome microarray can not only measure gene expression and obtain differentially expressed genes more accurately than traditional microarray, but also perform alternative splicing analysis that traditional chips can not complete. By calculating the expression of the probe set corresponding to each exon, we can obtain the expression of exon-level. By synthetically calculating the expression of all exons in a gene, we can obtain the expression at gene-level. By comparing the sample data of two or more different experiments, we can detect the difference of exon expression of the same gene under different experimental conditions, and then calculate the alternative splicing sites. CD Genomics exome microarrays cover specific functional exon variations selected from exon groups and genome-wide sequences of more than 10,000 individuals. Exons contain markers representing different continent groups, as well as a variety of common diseases, such as type II diabetes, cancer, metabolic and psychiatric disorders. CD Genomics has a variety of separate exon chips available to support various types of research aimed at finding functional-related disease associations. In addition, custom markers can be added to specific genome regions with higher density to focus on target populations or disease-related variants. You can use this exome microarray to gain new insights from the past genotyping microarray or conduct new research to find functional correlations. Our microarrays cover a wide range of common and rare variations in the human genome, as well as coding variations within the genome, providing the greatest potential for new insights into potential disease pathways. You can add up to 200,000 custom tags. CD Genomics microarrays can cover exons to the greatest extent and are suitable for new genotyping research. We have developed a powerful and high-density platform to provide full-exon solutions helping customers discover relationships between about 30,000 genes and more than 100,000 proteins. With CD Genomics exon microarrays, you can identify changes in gene and exon expression in a single experiment, thus capturing small but vital biological changes. Our bioinformatics system helps customers analyze gene expression data and alternative splicing markers simultaneously, which greatly improves the efficiency of laboratory work. CD Genomics exon solution can detect both low and high abundance expression products in a wide dynamic range, accurately reflect the whole expression level and ensuring reproducible results.

Human Exome Microarray Analysis Process

Fig. 1 Human Exome Microarray Analysis Process

DNA Sample requirements

• Amount: no less than 2 ug for each sample;
• Concentration: no less than 50ng/ul;
• Purity: OD 260/280 should be 1.7 ~ 1.9;
• Quality: genome should be integral, no degradation, no RNA contamination or other genomic DNA contamination;
• Preservation and transportation: samples should be transported at low temperature (- 20℃) and sealed with sealing film to prevent contamination.

CD Genomics offers high-quality services in human exome microarrays. We can provide conventional microarrays, customized microarrays and semi-customized microarrays services of exon analysis. With a large and well-equipped scientist team, we are dedicated to collaborating with our clients around the world to meet your specific requirements. We have comprehensive capacity and capabilities to provide a broad and integrated portfolio of laboratory and manufacturing services. If you are interested in our services, please contact us for more details.

Reference

1. Coleman J R I, et al. (2005). "Quality control, imputation and analysis of genome-wide genotyping data from the Illumina HumanCoreExome microarray." Briefings in Functional Genomics, 15 (4).