Plant Genotyping Microarray

CD Genomics is a leading global company who can provide the best solution for worldwide customers in the field of DNA microarray. We have successfully accomplished many projects in DNA microarray for genotyping. We guarantee the finest results for our customers all over the world.

For botanical research, genome-wide SNP chips are mainly used in Genome-wide association study, genetic map construction, population evolution analysis and so on. Although the technology of discovering molecular markers based on Next Generation Sequencing (NGS) is very mature, it is still time-consuming, expensive and challenging to conduct high-throughput data analysis, especially for plants with high heterozygous genome characteristics. With the features of fast, accurate and cheap, DNA microarray still has the advantages over NGS which can not be replaced. The advantages of DNA microarray mainly manifested in the following aspects: (1) DNA microarray for polyploid genotyping is far better than NGS; (2) The mutation sites of DNA microarray are fixed. In the following GWAS research in breeding and transformation applications, DNA microarray is easier to standardize; (3) Compared with NGS, DNA microarray results are stable, fast and easy to analyze. The results can be used for quantitative trait loci (QTL) analysis to identify how the gene expression leads to phenotypic differences. Hence, CD Genomics provides Plant Genotyping Microarray with various plant variants to validate the SNP genotyping.

Fig. 1 Identification of SNP markers and the development process of Plant Genotyping Microarray. (Li, XL, et al. 2019)

We can offer seamless and high-quality Plant Genotyping Microarray designs and construction services as well as Plant Genotyping Microarray test services. Custom DNA microarray services allow genotyping studies to identify variations associated with desired phenotypic traits, which can be used to identify new markers associated with a large number of species. Our laboratory plant SNP genotyping services include spermatophyte and sporophyte, including but not limited to the following species: Thale Cress, Barley, Wheat, Rice, Maize, Cotton, Tobacco, Rape, Tomato, Alfalfa, Brassica, Cherry, Peach, Soybean, Pepper, Apple, Pear, Grape, Oat, Potato, Sunflower, etc.

Sample requirements

• Sample type: fresh tissue or DNA sample;
• Amount: no less than 100mg tissue blocks or sections/ at least 1 ug DNA for each sample;
• Concentration: 0.5-50ng/µl;
• Purity: OD 260/280 should be 1.7 ~ 1.9;
• Quality: genome should be integral, no degradation, no RNA contamination or other genomic DNA contamination;
• Preservation and transportation: samples should be transported at low temperature (- 20℃) and sealed with sealing film to prevent contamination.

CD Genomics offers high-quality services in genome SNP genotyping. With a large and well-equipped scientist team, we are dedicated to collaborating with our clients around the world to meet your specific requirements. We have comprehensive capacity and capabilities to provide a broad and integrated portfolio of laboratory and manufacturing services. If you are interested in our services, please contact us for more details.

Reference

1. Li, XL, et al. (2019). "Development of an integrated 200K SNP genotyping array and application for genetic mapping, genome assembly improvement and GWAS in pear (Pyrus)." Plant Biotechnol J.