Serial Section Microarray Service
As a leading supplier in the field of microarrays, CD Genomics is committed to designing and developing various effective biological microarray products for non-clinical research and providing corresponding testing services. Our microarray test platform provides a full range of microarray-based analysis and measurement services for obtaining three-dimensional information in pathophysiology research to help our global customers conduct related research and reduce research costs. We are confident to provide you with high-quality products and high-quality services to help scientific research.
Serial Sectioning and Animal Models
The tissues and organs of experimental animals usually have complex three-dimensional structures, such as the brain, lungs, and vasculature. Conventional histological techniques provide good planar resolution and can be used to evaluate complex biological events on a two-dimensional surface of tissues and organs, but they do not provide three-dimensional information. This limitation may affect the understanding of complex tissue pathologies in health and disease. Therefore, histological technology has expanded the way of displaying three-dimensional information. Continuous slicing solves this problem to a certain extent. After taking materials along a certain direction of tissues and organs, keep the same thickness for continuous slices. These continuous tissue sections are arranged on a slide in a certain format to provide three-dimensional distribution information in this direction. Take the vasculature as an example. Microvessels constitute the complex distal end of the vascular tree, and their structure is essential to ensure optimal delivery of oxygenated blood in the tissue. The microvascular structure is three-dimensional in nature because the blood vessels are arranged in a highly branched network throughout the tissue. Therefore, understanding the organization of the microvascular network and its rearrangement in the pathological process may be crucial for analyzing the basis of organ dysfunction during disease. Although conventional two-dimensional histology can provide planar information for identifying the components of blood vessel walls, it cannot determine three-dimensional architectural information.
Fig 1. The connection between 2D histology and 3D histology. (Xu Y, et al. 2015)
Service
We developed this microarray service based on the continuous splicing method. This service is mainly customized microarrays for single organs. This service is very helpful for the research of atherosclerosis, fibrotic diseases, and vascular diseases. At the same time, there is also a need to obtain some three-dimensional structural information in the cancerous tissues of most organs. Our service process is to receive tissue samples, optional embedding, slice design, microarray design & printing, and optional histopathological testing. You can choose to send us fresh samples or fixed samples. After receiving the sample and forming the embedded block, we will communicate with you the size and direction of the slice. Later, we will provide you with suggestions for microarray design, including core size, number of cores and arrangement. After the microarray is printed and produced, we also provide optional histopathology services, as well as subsequent image acquisition and data processing.
Fig 2. Serial section tissue microarray service process.
Our Benefits
- Miniaturized design. This can save tissue blocks, a single model or a microarray composed of 2-4 consecutive slices
- Homogenization. This can ensure the consistency of human error in each part of the subsequent histopathological processing
- Save time, space and labor costs
CD Genomics provides high-quality microarray tools and convenient detection services for obtaining three-dimensional histopathological information in diseases. We have a large and well-equipped team of scientists who are committed to cooperating with researchers from all over the world. By choosing outsourcing testing services, your advantage is that you do not need to consider any other expenses, such as equipment, laboratory space, supervision or management. This convenient method can quickly turn around and provide reliable results, thus saving valuable time and resources. Please contact us for more detailed information.
Reference
- Xu Y, Pickering J G, Nong Z, et al. A method for 3D histopathology reconstruction supporting mouse microvasculature analysis[J]. PloS one, 2015, 10(5): e0126817.
Our products and services are for research purposes ONLY. We do not perform any private testing services that are not for laboratory use.
