As a leading service provider in the field of microarrays, CD Genomics is committed to the design and development of various effective biological microarrays for scientific research and non-clinical research. Our microarray test platform provides a full range of microarray-based transcription profiling and sRNA analysis and testing services to help our global customers conduct relevant research and reduce research costs. With our time-tested capabilities and regulatory expertise, we are confident to provide quality services in related testing services to escort your research.
Microarray and sRNA Expression Profiling
Small regulatory RNA (sRNA) is widely distributed in archaea, bacteria, and eukaryotes. It is a new type of endogenous non-protein coding RNA with a length of 21-35 nucleotides. sRNA mainly includes microRNAs (miRNAs), small interfering RNAs (siRNAs), small nucleolar RNAs (snoRNAs), siRNAs, Piwi-interacting RNAs (piRNA). Bacteria contain a variety of sRNAs, some of which have "housekeeping" functions, while other types of sRNAs play a regulatory role in gene expression. The cis-encoded RNA regulator is usually a part of the mRNA, located in front of the protein-coding region, and changes the expression of the corresponding protein by binding to small metabolites. Other regulatory RNAs are transcribed in trans from a separate promoter located in the intergenic region (IGR) or the opposite strand of the protein-coding region (ie, antisense RNA). The trans-encoded sRNA targets mRNA through short and imperfect sequence complementation, thereby regulating the expression of its target, and plays an important role in controlling bacterial stress response and adapting to changing environments.
Fig 1. sRNA search and characterization. (Haning K, et al. 2014)
Service Process
The use of microarrays to discover the function of sRNA requires simultaneous analysis of non-coding sRNA and protein-coding transcripts (i.e. mRNA). Our sRNA microarray service is optimized for the detection of small transcripts. The probe design adds many trans-sRNA complementary probes to the detection coding sequence, and the detection range is expanded on the basis of the established transcription map. Our sRNA microarray service currently mainly targets model microorganisms such as E. coli. If you are interested in sRNA analysis of other model organisms, you can also choose to order sRNA microarray printing services. We have designed multiple probes to detect the known trans-encoding sRNA transcripts of the samples. The data comes from the open transcript database and our own library. The specific service process is customized service content, microarray design and printing, microarray detection, and data analysis.
Fig 2. sRNA global analysis service process.
Our Features
- Comprehensive transcription profile analysis, covering a wide range;
- Reliable and fast process, shorten the cycle;
- Personalized service, providing a variety of options;
- Complete experimental platform, industry-leading data quality.
CD Genomics provides microarray-based detection services for transcriptomics and sRNA analysis research. We have a large and well-equipped team of scientists who are committed to cooperating with researchers from all over the world to meet customer requirements. Customers can directly contact our employees and provide timely feedback on their inquiries. If you are interested in our one-stop solution service, please contact us for more detailed information.
References
- Ahmad R, Hansen G Å, Hansen H, et al. Prediction, microarray and northern blot analyses identify new intergenic small RNAs in Aliivibrio salmonicida[J]. Journal of molecular microbiology and biotechnology, 2012, 22(6): 352-360.
- Haning K, Cho S H, Contreras L M. Small RNAs in mycobacteria: an unfolding story[J]. Frontiers in cellular and infection microbiology, 2014, 4: 96.
- Ruiz-Larrabeiti O, Plágaro A H, Gracia C, et al. A new custom microarray for sRNA profiling in Escherichia coli[J]. FEMS microbiology letters, 2016, 363(13).
Our products and services are for research purposes ONLY. We do not perform any private testing services that are not for laboratory use.
