CD Genomics is a leading global company who can provide the best solution for worldwide customers in the field of RNA microarray. We have successfully accomplished many projects in transcriptome analysis for both human, plant and animal with gene expression microarrays. We guarantee the finest results for our customers all over the world.
Transcriptome microarray is commonly used for revealing the molecular mechanism of biological phenomena or disease by comparing gene expression differences in high-throughput research. Gene expression profiles can be quickly obtained by using transcriptome microarray, which is widely used in basic research of biology and medicine, disease diagnosis, new drug development, environmental protection and many other aspects1. Transcriptome Microarray is the earliest platform to detect differentially expressed genes in the whole genome from the perspective of high throughput. Phenotypic abnormalities are rarely the result of changes in the expression of a single gene. Therefore, it is essential to generate a comprehensive expression profile in the study of normal biological processes and disease processes. In addition, important changes in expression, such as the use of differentially expressed exons caused by selective splicing events, may be masked in the analysis of gene horizontal profiles. Microarrays provide a great advantage in detecting millions of different sequences simultaneously, which makes the technology unaffected by problems encountered in detecting and measuring low-abundance transcripts or rare selective splicing events.
To facilitate transcriptome analysis and gene expression, CD Genomics provides comprehensive services for transcriptome detection and analysis. Our transcriptome microarrays show excellent performance in terms of data repeatability, average CV, and consistency with qPCR. Our probes have high sensitivity and specificity. The Transcriptome Microarray has a high detection rate, which is conducive to capturing low abundance genes that are important for life activities. At the same time, with the timely updating of the information base, the gene information updates more completely, and can be continuously upgraded to advanced chip versions. Our transcriptome microarrays are updated in time with the information database, and the genetic information is updated more completely. Customers can constantly upgrade to the advanced array versions. Our transcriptome microarray services include both animals and plants. Some available species are: human, arabidopsis, maize, rice, tobacco, cattle, chickens, dogs, horses, pigs, sheep, rabbits, monkeys, frogs, salmon, zebrafish, nematodes, mosquitoes, fruit flies, yeast, Escherichia coli,magnaporthe grisea, et al. In addition, we also provide flexible customization solutions for more species.
Technical process of Transcriptome Microarray
Fig. 1 Technical process of Transcriptome Microarray
RNA sample requirements
- Purity: OD 260/280 should be 1.9 ~ 2.2 and DNA should be clearly removed;
- Integrity: RNA integrity number (RIN) (≥7) and 28S/18S (≥0.7);
- Concentration: no less than 100ng/µl;
- Amount: no less than 2µg;
- Preservation and transportation: RNA is stored in freezing tubes and transported with dry ice or liquid nitrogen.
Process of Transcriptome Microarray data analysis
Fig. 2 Process of Transcriptome Microarray data analysis
CD Genomics offers high-quality services in Transcriptome Microarray. With well-equipped instruments and experienced scientist team, we are dedicated to collaborating with our clients around the world to meet your specific requirements. We have comprehensive capacity and capabilities to provide a broad and integrated portfolio of laboratory and manufacturing services. If you are interested in our services, please contact us for more details.
Reference
- Shanrong Z, et al. (2014). "Comparison of RNA-Seq and Microarray in Transcriptome Profiling of Activated T Cells." PLoS ONE, 9 (1), e78644.
