Transfected Cell Microarray Service

As a trusted supplier in the field of microarrays, CD Genomics is committed to designing and developing various effective biological microarray products and providing corresponding testing services. Our microarray detection platform provides a full range of microarray-based analysis services to help our customers reduce costs while using advanced technologies for related research. We are confident to provide you with high-quality products and high-quality services to accelerate the output of research results.

Cell-based Microarray Technology

Cell-based arrays are a powerful technology platform for performing high-throughput screening of arrays. Living cell microarray (LCMA) is an efficient cell screening system that is used to characterize cell-cell interactions, the interaction of cells with their microenvironment, and their response to applied stimuli, as well as an in-depth understanding of molecular cellular mechanisms. Since the number of cells required for each spot is small, the cell microarray can use primary cells and stem cells and provide resolution close to the single-cell level. For example, a transfected-cell array (TCA) is to locate ("spot") different nucleic acids together with transfection reagents at designated positions on the surface of a support made of a specific material. Then cover the surface with cells transfected in the "spot" area. In addition to various traditional static designs (2-D format), a microfluidic microarray system has also been established. Another format is a microarray (3-D format) composed of three-dimensional cell structures, ranging from cell spheres to cells encapsulated in hydrogels. These systems provide a microenvironment similar to the in vivo and are preferably used for research in cell physiology, cytotoxicity and drug screening.

Fig 1. Spotted cell microarray technology. (Yarmush M L, et al. 2009)

Service

The transfected cell array is suitable for rapid and systematic high-throughput screening of genes that cause the desired phenotype or encode the target product. We provide a fixed TCA service. The service uses high-density printing of genomic materials (such as expression vectors and/or siRNA molecules) with lipofection reagents onto a support surface of specific materials, and then dry storage of the resulting high-density nanodot arrays. After receiving the customer's sample, the microarray is hydrated, and a single cell type is uniformly seeded in the entire array to form a confluent monolayer of cells. Only the cells growing on the top of the spots are transfected, resulting in the expression or silencing of specific proteins in spatially different cell populations. Cell-specific bioassays can be used to detect the phenotypic effects of this "reverse transfection" of hundreds of genes. Our cell microarray experiment service always uses automated microscopes and image-based analysis to quantify results. In addition, detecting protein interactions on transfected cell arrays allows for the screening and selection of molecules such as small peptides or siRNA aptamers in vivo, which can be inhibited by co-transfecting libraries of such small molecules in cells expressing the target protein. interaction. We use cell arrays as a platform to perform high-throughput analysis of protein-DNA and protein-protein interactions by combining transfected cell arrays with mammalian one-hybrid and two-hybrid systems.

Fig 2. Flow chart of transfection cell microarray service.

Technical Advantages

• Cell behavior analysis. Basic cell biology research;
• Drug testing. Complex drug testing procedures;

CD Genomics provides high-quality cell array tools and convenient detection services. We have a large team of scientists with a solid academic foundation and cooperate with researchers in basic biology and biomedical projects from all over the world. The advantage of choosing an analytical testing outsourcing testing service is that there is no need to consider any other costs (such as equipment and laboratory space), and it saves supervision or management. This advanced technology can quickly turn around and provide reliable results, thereby saving valuable time and resources. Please contact us for more detailed information.

References

1. Yarmush M L, King K R. Living-cell microarrays[J]. Annual review of biomedical engineering, 2009, 11: 235-257.
2. Kaylan K B, Kourouklis A P, Underhill G H. A high-throughput cell microarray platform for correlative analysis of cell differentiation and traction forces[J]. JoVE (Journal of Visualized Experiments), 2017 (121): e55362.