tRNA and Expression Analysis
As a leading service provider in the field of microarrays, CD Genomics is committed to the design and development of various effective biological microarrays for scientific research and non-clinical research. Our microarray test platform provides a full range of microarray-based tRNA analysis services to help our global customers conduct relevant research and reduce research costs. With our time-tested capabilities and regulatory expertise, we are confident to provide quality services in related testing services to escort your research.
Microarray and tRNA Expression Profiling
More than 450 transfer RNA (tRNA) genes have been annotated in the human genome, constituting 274 different sequences. These tRNA genes are scattered throughout the genome and are present on all chromosomes except the Y chromosome. In addition, there are 22 other tRNA genes in human mitochondrial DNA. Approximately 473 human tRNAs are divided into 49 families to decode the 21 amino acids (20 standard amino acids and selenocysteine) specified by the genetic code. Studies have shown that the expression of tRNA is tissue-specific, and this heterogeneity seems to play a different role in regulating translation and other processes in vertebrate organisms. For example, differences in the relative abundance of tRNA subtypes have been found to affect protein expression levels in bacteria and yeast. The tRNAs that decode amino acids with similar chemical properties show cooperative expression patterns in different tissue types, which helps to better understand the biological process of protein translation.
Fig 1. The tRNA Microarray. (Dittmar K A, et al. 2006)
Service Process
The use of microarray methods to reliably quantify tRNA levels in human samples is a technology developed in recent years, which can help decode the relationship between tRNA abundance and codon distribution and predict the regulation of gene expression. We use a microarray method to quantify tRNA based on fluorescent dye labeling technology. Remember that the service process is to receive samples and extract total RNA, and at the same time determine the quality of RNA. Then reverse transcribed the sample into cDNA to build a sample library and label the sequence with fluorescent dyes. Finally, use prefabricated microarrays or customized printed microarrays to detect and harvest data. The probe design of our tRNA array is based on the Genomic tRNA Database and our own database.
Fig 2. Microarray-based tRNA analysis process.
Our Features and Benefits
- Provide personalized service, choose our prefabricated microarray products or customized microarray printing products;
- Reliable, professional and fast inspection process;
- Have a professional bioinformatics team to provide industry-leading data quality.
CD Genomics provides microarray-based detection services for tRNA expression research. We have a large and well-equipped team of scientists who are committed to cooperating with researchers from all over the world to meet customer requirements. Customers can directly contact our employees and provide timely feedback on their inquiries. If you are interested in our one-stop solution service, please contact us for more detailed information.
References
- Dittmar K A, Goodenbour J M, Pan T. Tissue-specific differences in human transfer RNA expression[J]. PLoS Genet, 2006, 2(12): e221.
- Schwartz M H, Pan T. Determining the fidelity of tRNA aminoacylation via microarrays[J]. Methods, 2017, 113: 27-33.
- Grelet S, McShane A, Hok E, et al. SPOt: A novel and streamlined microarray platform for observing cellular tRNA levels[J]. PloS one, 2017, 12(5): e0177939.
Our products and services are for research purposes ONLY. We do not perform any private testing services that are not for laboratory use.
