CD Genomics is a leading global company who can provide the best solution for worldwide customers in the field of RNA microarray. We have successfully accomplished many projects in miRNA profiling, lncRNA, tRNA, tRF&tiRNA, snoRNA, mRNA and circRNA microarrays. We guarantee the finest results for our customers all over the world.Our RNA Microarray Services Platform including but not limited to:
Transcriptome Microarray is the earliest platform to detect differentially expressed genes in the whole genome from the perspective of high throughput. Phenotypic abnormalities are rarely the result of changes in the expression of a single gene. Therefore, it is essential to generate a comprehensive expression profile in the study of normal biological processes and disease processes. CD Genomics provides comprehensive services for transcriptome microarrays with excellent performance in terms of data repeatability, average CV, and consistency with qPCR.
The miRNA is highly conservative with time-sequential and tissue-specific expression. It guides the RNA-induced silencing complex (RISC) to degrade or inhibit the translation of mRNA by pairing with target genes, thus regulating gene expression at the transcriptional or post-transcriptional level. miRNA is involved in many physiological processes and abnormal expression of miRNA often leads to disease. CD Genomics provides miRNA profiling microarrays to detect the expression level of microRNAs, screen differentially expressed microRNAs and predict differentially targeted microRNAs.
lncRNAs can regulate gene expression at epigenetic, transcriptional and post-transcriptional levels and are involved in many important regulatory processes. They are also closely related to the occurrence, development and prevention of human diseases. Long non-coding RNAs play an important role in the evolution of new Although more lncRNAs have been discovered, their functions are mostly unclear. CD Genomics provides lncRNA microarray service platforms to screen differential lncRNA related to diseases or specific phenotypes.
tRNA is an important messenger between genes and proteins. Changes in tRNA expression profiles have significant effects on many cell physiological processes. A series of life activities are accompanied by changes in tRNA levels. Therefore, the study of tRNA spectrum is very important for further understanding the physiological and pathological processes of human body. CD Genomics provides tRNA microarray services to facilitate researchers to quickly detect the expression of tRNA modification-related proteins.
As sncRNAs, tRFs and tiRNAs perform various biological functions, the composition and content of tRFs & tiRNAs are highly dependent on cell subtypes and closely related to various pathological conditions. They are ubiquitous in biological fluids far more than microRNA, making them promising molecular markers. Their characteristic expression in tumors can make them valuable markers for the diagnosis of tumors and can be used to guide clinical treatment. CD Genomics offers a broad and integrated portfolio of tRF & tiRNA Microarray services.
snoRNA is mainly located in the nucleolus and related to the processing and modification of rRNA. Recent studies showed that snoRNA is generally overexpressed in tumors and involved in the process of disease. snoRNA is involved in many biological processes and mainly works through snoRNPs complex. snoRNAs are stable in blood, sputum and urine, which have great prospects as molecular diagnostic markers of diseases. Nowadays, snoRNA has attracted much attention in pathology studies, especially cancer. We can provide a series of snoRNA Microarray design and construction solutions and customized services to meet your special requirements.
In recent years, circRNA has become a hot field in biological research, especially in disease research. circRNA is a new type of RNA, which has a closed ring structure and exists in a large number of eukaryotic transcriptomes. circRNA has a specific expression pattern in many diseases and plays an important biological function. circRNA's tissue specificity, disease specificity, sequence specificity, and high stability bestow them advantages as biomarkers of clinical diseases. Compared with second-generation sequencing, microarrays can detect more circRNAs, including circRNA with low expression abundance. We develop circRNA microarrays to detect genome-wide circRNA effectively.
High-throughput next-generation sequencing (NGS) and gene microarray can both analyze the number of copies of genes in the transcriptome and different spliceosomes. They have been widely used for a long time, and microarray has been used more widely and earlier. Microarrays have been the backbone of genome expression analysis since the mid-1990s. Those studying gene expression patterns thought of using chips. Microarray analysis relies on known genomic information, which is the biggest limitation of the technology. RNA-seq transcriptome analysis is unbiased and can reveal new splicing points, small RNAs and new genes missing from microarrays. As the cost of sequencing plummets, RNA-seq has become a popular transcriptome analysis method. Although RNA-seq has many advantages, many researchers continue to use RNA microarrays. Compared with RNA-seq, RNA microarray is more mature, especially for large sample size research. RNA microarray is more widely used in clinical research, and its data processing is faster and simpler. It can provide highly consistent data using mature analysis software. By analyzing hundreds of samples, the expression characteristics of genes and microRNAs have been endowed with clinical diagnostic value. You can use RNA microarrays to quickly screen a large number of samples, and then use these results to guide RNA-seq. In addition, the chip can also be used to verify the data of RNA-seq.
RNA microarray technical process
- RNA is extracted from the samples, transformed into cDNA, and labeled with fluorescent markers.
- The labeled cDNA fragments are hybridized with the nucleic acid on the chip.
- Scan the microarray to detect the fluorescence level of each spot and obtain the gene expression level.
RNA sample requirements
- Purity: OD 260/280 should be 1.9 ~ 2.2 and DNA should be clearly removed;
- Integrity: RNA integrity number (RIN) (≥7) and 28S/18S (≥0.7);
- Concentration: no less than 100ng/µl;
- Amount: no less than 2µg;
- Preservation and transportation: RNA is stored in freezing tubes and transported with dry ice or liquid nitrogen.
CD Genomics offers high-quality services in RNA Microarray. With well-equipped instruments and experienced scientist team, we are dedicated to collaborating with our clients around the world to meet your specific requirements. We have comprehensive capacity and capabilities to provide a broad and integrated portfolio of laboratory and manufacturing services. If you are interested in our services, please contact us for more details.
Reference
- Nazarov PV, et al. (2017). "RNA sequencing and transcriptome arrays analyses show opposing results for alternative splicing in patient derived samples." BMC Genomics, 18(1), 443.
