Transcriptomics Profiling with Microarray
CD Genomics is committed to providing microarray-based transcriptomics analysis solutions for global customers in the field of microarrays. We have completed many gene expression analysis projects in cooperation with many scientific research institutions, universities, companies and non-clinical research organizations. We will return the trust of customers with excellent product quality, and provide customers all over the world with the most satisfactory products and services.
Understanding the control of gene expression is critical to our understanding of the relationship between genotype and phenotype because gene expression analysis helps researchers discover new RNA forms and variants. The need for a reliable assessment of transcript abundance in biological samples has prompted the development of new analytical techniques. Microarray technology has been developed to efficiently analyze RNA expression. Although the analysis of small non-coding RNAs (ncRNAs) is challenging because of their short length, large sequence differences, and large variations in GC content, resulting in very different hybridization characteristics. LNA-modified oligonucleotide probes have been used to enhance and standardize the melting temperature (Tm) of capture probes, which allows sensitive analysis of small ncRNAs regardless of their sequence.
Fig 1. Microarray experimental design: Comparison of gene expression in two human cell lines using UHRR as a common reference sample. (Novoradovskaya N, et al. 2004)
The entire service content of our transcription analysis microarray platform includes the separation and labeling of small ncRNAs, the use of semi-automatic hybridization equipment and modified oligonucleotide probe microarrays, and data analysis services. When comparing two or more related biological conditions, RNA microarrays can be widely used to identify regulated genes, pathways, or gene networks in various cells and tissues. This method provides an in-depth understanding of biological mechanisms or cellular processes, such as cell cycle progression, embryonic development, cell fate determination, hormone-responsive gene regulation programs, and drug or disease model-mediated gene expression changes. Our transcriptome microarray analysis service can analyze nuclear transcripts and mitochondrial transcripts as well as a series of ncRNAs related to cancer regulation. In particular, the service is based on characteristic LNA modified nucleotide probes, and all semi-customized and fully customized personalized products are used to meet the needs of customers for different projects. Our experimental team will design this part of the experimental program for you, and strictly implement the quality control link in the follow-up test to ensure the final data quality.
Fig 2. Comprehensive transcriptome microarray analysis service process.
Available Services
| Key Points |
Description |
| Gene Expression |
Microarray technology has been used as a means of generating transcriptome information. Analyzing the biological significance of different processing methods or processes in developing organisms through microarray hybridization can help us better decipher life activities. Our transcriptome analysis microarray service performs high-throughput analysis of gene expression. |
| sRNA Profiling |
Small regulatory RNA (sRNA) is a new type of endogenous non-coding RNA that plays a regulatory role in gene expression. Our sRNA microarray service enables simultaneous analysis of non-coding sRNA and protein-coding transcripts. |
| microRNA Profiling |
miRNA is a short non-coding RNA molecule that plays a role in the post-transcriptional regulation of gene expression. Many miRNAs are highly conserved and have unique tissue-specific, developmental stage-specific, or disease-specific patterns. Microarrays provide convenience for basic research and applied research related to miRNA functions. Our miRNA expression analysis service aims to study the differences in miRNA biogenesis, tissue distribution, normal and abnormal miRNA expression. |
| LncRNA Profiling |
Long non-coding RNA (lncRNA) is a transcript that has no protein-coding potential and plays a key role in regulating gene expression. Thousands of lncRNAs have been identified in invertebrates, some of which have been shown to have certain biological functions. Our lncRNA array analysis service is aimed at the systematic analysis of lncRNA and the expression analysis of related protein-coding mRNA. |
| snoRNA |
Small nucleolar RNA (snoRNA) is a key component of snoRNP (small nucleolar ribonucleoprotein) particles, a highly conserved ribonucleoprotein complex that is essential for ribosome biogenesis. The snoRNP production defect caused by the mutation of the snoRNA gene or its related protein-coding gene may have a profound impact on cell metabolism and proliferation. We provide snoRNA analysis and testing services. |
| Circular RNA |
Circular RNA (circRNA) is a universal and diverse endogenous non-coding RNA (ncRNA) that has the function of regulating gene expression. Microarray tools can be used as a more sensitive, effective and cost-effective circRNA expression profiling technique. We provide genome-wide circRNA expression profile analysis services. |
| tRNA |
A large number of tRNA genes have been annotated in the human genome, which are important transporters of protein translation. The expression of tRNA has certain tissue specificity, and this heterogeneity seems to play a different role in regulating translation and other processes of vertebrate organisms. We use microarray technology to provide analysis services for tRNA expression analysis. |
| tiRNA and tRF |
tiRNA (half of tRNA) and tRF (fragment of tRNA) are small non-coding RNA (ncRNA) derived from tRNA with different functions and are involved in expression regulation. We provide microarray technology services for the detection of tRF and tiRNA. |
| piRNA |
PIWI Interacting RNA (piRNA) is a small single-stranded non-coding RNA that interacts with the piwi protein and acts as a complex to regulate cell activity. We designed piRNA microarrays for humans and laboratory animals to evaluate the global expression profile of piRNA. |
| T-UCR |
Transcriptional ultra-conserved region (T-UCR) transcript is a new type of lncRNA transcribed from the ultra-conserved region (UCR). T-UCRs formed by UCR transcription show a ubiquitous and tissue-specific pattern at the expression level. Our T-UCR microarray service can be used to analyze the expression of T-UCR. |
| Disease-specific lncRNA |
The coding sequences of some lncRNAs related to disease signaling pathways are usually located on or near protein-coding genes that are critical to the pathway or disease state. The role of a single lncRNA in disease does not seem to be all, and many biological functions may be affected by the combined effects of multiple lncRNAs. We provide microarray technology services for rapid analysis of disease-related lncRNAs. |
| Cancer-related lncRNA |
LncRNAs have different roles in different types of cancer, but they are accompanied by some important signal pathways. Microarrays are very helpful for the rapid detection of these fragments. We provide microarray services for rapid analysis of lncRNA related to cancer-related signaling pathways. |
| Cancer-related miRNA |
The information exchange between cancer cells and the surrounding microenvironment is considered to be the basis of tumorigenesis, progression, invasion and final metastasis. The communication between tumor microenvironment cells and cancer cells is achieved through miRNAs. We provide a unique customized microarray service to analyze the expression of cancer-related miRNAs. |
| Mitochondrial Transcriptomics |
Mitochondrial dysfunction is related to many pathological conditions in humans. It is regulated by the mitochondrial genome (mtDNA) and/or nuclear genes encoding mitochondrial proteins. Our mitochondrial transcriptome analysis service can be used to analyze known nuclear and mitochondrial genes related to mitochondrial structure and function, as well as genes known to cause mitochondrial dysfunction. |
| Mt-lncRNA |
Like nuclear DNA, mitochondrial DNA is also regulated by epigenetic factors, especially DNA methylation and non-coding RNA (ncRNA). We provide microarray tools for mt-ncRNA analysis. This tool can be used to analyze the role of non-coding RNA in mitochondrial epigenetics. |
| Protein Binding |
Protein-DNA binding is the key to gene regulation specificity. DNA microarray-based protein binding microarrays can be used to identify the sequence specificity of in vitro DNA binding sites of transcription factors and other DNA binding proteins or synthetic compounds. Our microarray platform provides protein-binding microarray services based on in-situ synthesis. |
| Peptide-RNA Interaction |
Microarray technology has always been a powerful tool for high-throughput and rapid analysis of RNA-peptide interactions. Microarray-based ligand screening provides a fast, direct, and high-throughput platform that can identify molecules that bind to RNA. We provide RNA-peptide interaction analysis services based on high-quality peptide microarrays. |
| Transcriptome Expression |
Transcriptome gene expression chip is the first platform to detect differentially expressed genes in the whole genome from a high-throughput perspective. At present, it is widely used in many research fields such as gene expression detection, gene function research, new gene identification, drug target screening, drug action mechanism and toxicology, disease-related genes and biomarkers. We provide customized transcriptome gene expression chips for large-scale analysis of gene expression changes in specific biological objects during specific biological processes. |
CD Genomics provides high-quality commercial, customized and semi-customized microarray products and analysis&testing services based on microarray products. Our microarray test platform has well-equipped laboratories and a team of highly qualified scientists, and we look forward to cooperating with customers all over the world. We have comprehensive design and production capabilities, and can provide a broad and comprehensive combination of laboratory and manufacturing services. If you are interested in our products and services, please contact us for more details.
References
- Novoradovskaya N, Whitfield M L, Basehore L S, et al. Universal Reference RNA as a standard for microarray experiments[J]. BMC genomics, 2004, 5(1): 1-13.
- Sealfon S C, Chu T T. RNA and DNA microarrays[M]//Biological microarrays. Humana Press, Totowa, NJ, 2011: 3-34.
- Wang C, Gong B, Bushel P R, et al. The concordance between RNA-seq and microarray data depends on chemical treatment and transcript abundance[J]. Nature biotechnology, 2014, 32(9): 926-932.
Our products and services are for research purposes ONLY. We do not perform any private testing services that are not for laboratory use.
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